Bioprocess Innovation Leaders Summit Speakers

Jochen B. Sieck

Senior R&D Manager

We developed a combined process featuring N-1 perfusion in a novel expansion medium and high cell density cryopreservation (HCDC) in 250 mL frozen bags as seed train intermediates. Our results indicate that using an expansion medium specifically designed for N-1 perfusion can prepare the cells optimally for the final N-stage and increase cellular productivity while using low CSPRs.